Journal of Biomedical Advancement Scientific Research

Abstract

Background: Stem cell harvesting techniques historically require invasive methods to gather stem cells. Advancements in technology and research urge the need to find minimally invasive methods of stem cell harvesting.

Purpose: The primary purpose is to quantify the amount of stem cells harvested from peripheralveni puncture after blood flow restriction exercises. The secondary purpose of this research is to identify a minimally invasive method to harvest stem cells. The overall purpose is to determine the effects of blood flow restriction training with the upper extremity on systemic stem cell quantity post-training with a single bout of exercise while identifying a minimally invasive method of harvesting stem cells in significant quantities and being universally achievable by most individuals in the population.

Study Design: This study follows a controlled intervention design. All participants follow a structured protocol in controlled conditions. Quantitative data is measured and gathered to test against the hypothesis.

Methods: Participants enrolled in this study underwent a structured exercise protocol consisting of 3 bilateral upper extremity exercises. Each participant was physically evaluated for the ability to safely perform weighted exercises. A Delfi blood flow restriction device was used to maintain constant venous occlusion in the upper extremities to create a temporary localized hypoxic environment of the upper limbs. Venipuncture is performed at time points before, during, and after the exercise protocol for quantitative measurement of stem cells in the peripheral venous system.